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Journal of Bacteriology, March 2007, p. 2186-2189, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01645-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

YjjG, a dUMP Phosphatase, Is Critical for Thymine Utilization by Escherichia coli K-12{triangledown}

Bernard Weiss*

Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322

Received 23 October 2006/ Accepted 12 December 2006

Exogenous thymine must be converted to thymidine to enable a thyA (thymidylate synthase) mutant to grow. The deoxyribose in the thymidine comes from dUMP, which must first be dephosphorylated. The nucleotidase YjjG is critical for this step. A yjjG thyA mutant cannot use thymine for growth on a glucose minimal medium.

* Mailing address: Department of Pathology and Laboratory Medicine, Emory University, Whitehead Bldg., Rm. 141, 615 Michael St., Atlanta, GA 30322. Phone: (404) 712-2812. Fax: (404) 727-8538. E-mail: bweiss2{at}emory.edu.

{triangledown} Published ahead of print on 22 December 2006.

Journal of Bacteriology, March 2007, p. 2186-2189, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01645-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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