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Evidence for Type III Restriction and Modification Systems in Mycoplasma pulmonis

Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294

Received 27 October 2006/ Accepted 26 December 2006

Mycoplasma pulmonis possesses a cassette of genes that are predicted to code for type III restriction and modification (R-M) enzymes. Transposon disruption of a gene predicted to code for the endonuclease subunit of the enzyme resulted in loss of R-M activity. Genomic data indicate that the cassette was acquired by horizontal gene transfer and possibly located on a mobile element.

Published ahead of print on 5 January 2007.