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Journal of Bacteriology, March 2007, p. 2197-2202, Vol. 189, No. 6
0021-9193/07/$08.00+0     doi:10.1128/JB.01669-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evidence for Type III Restriction and Modification Systems in Mycoplasma pulmonis{triangledown}

Kevin Dybvig,* Z. Cao, C. Todd French, and Huilan Yu

Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294

Received 27 October 2006/ Accepted 26 December 2006

Mycoplasma pulmonis possesses a cassette of genes that are predicted to code for type III restriction and modification (R-M) enzymes. Transposon disruption of a gene predicted to code for the endonuclease subunit of the enzyme resulted in loss of R-M activity. Genomic data indicate that the cassette was acquired by horizontal gene transfer and possibly located on a mobile element.

* Corresponding author. Mailing address: Department of Genetics, 720 S. 20th St., KAUL 720, University of Alabama at Birmingham, Birmingham, AL 35294-0024. Phone: (205) 934-9327. Fax: (205) 975-4418. E-mail: dybvig{at}uab.edu.

{triangledown} Published ahead of print on 5 January 2007.

Journal of Bacteriology, March 2007, p. 2197-2202, Vol. 189, No. 6
0021-9193/07/$08.00+0     doi:10.1128/JB.01669-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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