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Journal of Bacteriology, March 2007, p. 2238-2248, Vol. 189, No. 6
0021-9193/07/$08.00+0     doi:10.1128/JB.01689-06

The Novel Transcription Factor SgrR Coordinates the Response to Glucose-Phosphate Stress{triangledown} ,{dagger}

Carin K. Vanderpool* and Susan Gottesman

Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892

Received 1 November 2006/ Accepted 21 December 2006

SgrR is the first characterized member of a family of bacterial transcription factors containing an N-terminal DNA binding domain and a C-terminal solute binding domain. Previously, we reported genetic evidence that SgrR activates the divergently transcribed gene sgrS, which encodes a small RNA required for recovery from glucose-phosphate stress. In this study, we examined the regulation of sgrR expression and found that SgrR negatively autoregulates its own transcription in the presence and absence of stress. An SgrR binding site in the sgrR-sgrS intergenic region is required in vivo for both SgrR-dependent activation of sgrS and autorepression of sgrR. Purified SgrR binds specifically to sgrS promoter DNA in vitro; a mutation in the site required for in vivo activation and autorepression abrogates in vitro SgrR binding. A plasmid library screen identified clones that alter expression of a PsgrS-lacZ fusion; some act by titrating endogenous SgrR. The yfdZ gene, encoding a putative aminotransferase, was identified in this screen; the yfdZ promoter contains an SgrR binding site, and transcriptional fusions indicate that yfdZ is activated by SgrR. Clones containing mlc, which encodes a glucose-specific repressor protein, also downregulate PsgrS-lacZ. The mlc clones do not appear to titrate the SgrR protein, indicating that Mlc affects sgrS expression by an alternative mechanism.

* Corresponding author. Present address: Department of Microbiology, University of Illinois at Urbana-Champaign, B213 CLSL, MC-110, 601 S. Goodwin Ave., Urbana, IL 61801. Phone: (217) 333-7033. Fax: (217) 244-6697. E-mail: cvanderp{at}life.uiuc.edu.

{triangledown} Published ahead of print on 5 January 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, March 2007, p. 2238-2248, Vol. 189, No. 6
0021-9193/07/$08.00+0     doi:10.1128/JB.01689-06




This article has been cited by other articles:

  • Horler, R. S. P., Vanderpool, C. K. (2009). Homologs of the small RNA SgrS are broadly distributed in enteric bacteria but have diverged in size and sequence. Nucleic Acids Res 37: 5465-5476 [Abstract] [Full Text]  
  • Wadler, C. S., Vanderpool, C. K. (2009). Characterization of homologs of the small RNA SgrS reveals diversity in function. Nucleic Acids Res 37: 5477-5485 [Abstract] [Full Text]  
  • Wadler, C. S., Vanderpool, C. K. (2007). From the Cover: A dual function for a bacterial small RNA: SgrS performs base pairing-dependent regulation and encodes a functional polypeptide. Proc. Natl. Acad. Sci. USA 104: 20454-20459 [Abstract] [Full Text]  


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