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Journal of Bacteriology, April 2007, p. 2945-2948, Vol. 189, No. 7
0021-9193/07/$08.00+0 doi:10.1128/JB.01723-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Ludwig-Maximilians-Universität München, Department Biologie I, Bereich Mikrobiologie, München, Germany
Received 8 November 2006/ Accepted 17 January 2007
Hydropathy profile analyses of the amino acid sequence of the quorum-sensing hybrid histidine kinase LuxN of Vibrio harveyi predict a periplasmic location of the N terminus. To test this, two-hybrid proteins consisting of LuxN and an N-terminally fused maltose-binding protein with or without a leader sequence were analyzed with regard to the enzymatic activities of LuxN, protease accessibility, and complementation of an Escherichia coli malE mutant. The results strongly support a periplasmic location of the N terminus, implying that LuxN is anchored with nine transmembrane domains in the cytoplasmic membrane.
Published ahead of print on 26 January 2007.
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