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Journal of Bacteriology, April 2007, p. 2945-2948, Vol. 189, No. 7
0021-9193/07/$08.00+0     doi:10.1128/JB.01723-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Quorum-Sensing Hybrid Histidine Kinase LuxN of Vibrio harveyi Contains a Periplasmically Located N Terminus{triangledown}

Kirsten Jung,* Tina Odenbach, and Melanie Timmen

Ludwig-Maximilians-Universität München, Department Biologie I, Bereich Mikrobiologie, München, Germany

Received 8 November 2006/ Accepted 17 January 2007

Hydropathy profile analyses of the amino acid sequence of the quorum-sensing hybrid histidine kinase LuxN of Vibrio harveyi predict a periplasmic location of the N terminus. To test this, two-hybrid proteins consisting of LuxN and an N-terminally fused maltose-binding protein with or without a leader sequence were analyzed with regard to the enzymatic activities of LuxN, protease accessibility, and complementation of an Escherichia coli malE mutant. The results strongly support a periplasmic location of the N terminus, implying that LuxN is anchored with nine transmembrane domains in the cytoplasmic membrane.

* Corresponding author. Mailing address: Ludwig-Maximilians-Universität, Department Biologie I, Bereich Mikrobiologie, Maria-Ward-Str. 1a, D-80638 München, Germany. Phone: 49 89 2180 6120. Fax: 49 89 2180 6122. E-mail: kirsten.jung{at}lrz.uni-muenchen.de.

{triangledown} Published ahead of print on 26 January 2007.

Journal of Bacteriology, April 2007, p. 2945-2948, Vol. 189, No. 7
0021-9193/07/$08.00+0     doi:10.1128/JB.01723-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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