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Journal of Bacteriology, April 2007, p. 3296-3301, Vol. 189, No. 8
0021-9193/07/$08.00+0     doi:10.1128/JB.01860-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Microarray Analysis of Transposon Insertion Mutations in Bacillus anthracis: Global Identification of Genes Required for Sporulation and Germination{triangledown} ,{dagger}

William A. Day Jr.,1* Suzanne L. Rasmussen,1 Beth M. Carpenter,1,{ddagger} Scott N. Peterson,2 and Arthur M. Friedlander1*

Bacteriology and Headquarters Divisions, United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702,1 Department of Microbial Genomics, The Institute for Genomic Research, Rockville, Maryland 208502

Received 11 December 2006/ Accepted 23 January 2007

A transposon site hybridization (TraSH) assay was developed for functional analysis of the Bacillus anthracis genome using a mini-Tn10 transposon which permitted analysis of 82% of this pathogen's genes. The system, used to identify genes required for generation of infectious anthrax spores, spore germination, and optimal growth on rich medium, was predictive of the contributions of two conserved hypothetical genes for the phenotypes examined.

* Corresponding author. Mailing address for William A. Day, Jr.: Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702. Phone: (301) 619-7176. Fax: (301) 619-2152. E-mail: william.day{at}amedd.army.mil. Mailing address for Arthur M. Friedlander: Headquarters Division, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702. Phone: (301) 619-7343. Fax: (301) 619-4922. E-mail: arthur.friedlander{at}amedd.army.mil

{triangledown} Published ahead of print on 2 February 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.

Journal of Bacteriology, April 2007, p. 3296-3301, Vol. 189, No. 8
0021-9193/07/$08.00+0     doi:10.1128/JB.01860-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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