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Role of DNA Repair by Nonhomologous-End Joining in Bacillus subtilis Spore Resistance to Extreme Dryness, Mono- and Polychromatic UV, and Ionizing Radiation

German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Research Group Photo- and Exobiology, Cologne, Germany,¹ German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany,² Genome Damage and Stability Centre, University of Sussex, Brighton BN1 9RQ, United Kingdom,³ Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899⁴

Received 4 January 2007/ Accepted 5 February 2007

The role of DNA repair by nonhomologous-end joining (NHEJ) in spore resistance to UV, ionizing radiation, and ultrahigh vacuum was studied in wild-type and DNA repair mutants (recA, splB, ykoU, ykoV, and ykoU ykoV mutants) of Bacillus subtilis. NHEJ-defective spores with mutations in ykoU, ykoV, and ykoU ykoV were significantly more sensitive to UV, ionizing radiation, and ultrahigh vacuum than wild-type spores, indicating that NHEJ provides an important pathway during spore germination for repair of DNA double-strand breaks.

Published ahead of print on 9 February 2007.

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