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Isolation of the rstA Gene as a Multicopy Suppressor of YjeE, an Essential ATPase of Unknown Function in Escherichia coli

Tracey L. Campbell, Claudia S. Ederer,^, Abdellah Allali-Hassani,^¶ and Eric D. Brown^*

Antimicrobial Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5

Received 24 January 2006/ Accepted 18 January 2007

YjeE is an essential ATPase in Escherichia coli whose cellular function remains uncharacterized. Using a genomic library, we have identified rstA as a multicopy suppressor of a conditional yjeE deletion strain. High-copy rstA is the first recorded suppressor for a lesion in yjeE, and this newly charted genetic interaction has the potential to be informative about the function, with further study of the interacting partners.

Published ahead of print on 9 February 2007.

T.L.C. and C.S.E. contributed equally to this work.

Present address: Klinik I für Innere Medizin, Klinikum der Universität zu Köln, Kerpener Straße 62, 50937 Köln, Germany.

^¶ Present address: Structural Genomics Consortium, Banting Building, University of Toronto, 100 College St., Toronto, Ontario, M5G 1L6, Canada.