JB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JB.00619-06v1
189/9/3479    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stork, M.
Right arrow Articles by Crosa, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stork, M.
Right arrow Articles by Crosa, J. H.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2007, p. 3479-3488, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.00619-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Transcription Termination within the Iron Transport-Biosynthesis Operon of Vibrio anguillarum Requires an Antisense RNA{triangledown}

Michiel Stork,{dagger} Manuela Di Lorenzo, Timothy J. Welch,§ and Jorge H. Crosa*

Department of Molecular Microbiology and Immunology L-220, Oregon Health and Science University, Portland, Oregon 97201-3098

Received 2 May 2006/ Accepted 23 February 2007

The iron transport-biosynthesis (ITB) operon in Vibrio anguillarum includes four genes for ferric siderophore transport, fatD, -C, -B, and -A, and two genes for siderophore biosynthesis, angR and angT. This cluster plays an important role in the virulence mechanisms of this bacterium. Despite being part of the same polycistronic mRNA, the relative levels of transcription for the fat portion and for the whole ITB message differ profoundly, the levels of the fat transcript being about 17-fold higher. Using S1 nuclease mapping, lacZ transcriptional fusions, and in vitro studies, we were able to show that the differential gene expression within the ITB operon is due to termination of transcription between the fatA and angR genes, although a few transcripts proceeded beyond the termination site to the end of this operon. This termination process requires a 427-nucleotide antisense RNA that spans the intergenic region and acts as a novel transcriptional terminator.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Immunology L-220, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd., Portland, OR 97201-3098. Phone: (503) 494-7583. Fax: (503) 494-6862. E-mail: crosajor{at}ohsu.edu

{triangledown} Published ahead of print on 2 March 2007.

{dagger} Present address: Department of Molecular Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

§ Present address: National Center for Cool and Cold Water Aquaculture, Agriculture Research Service/U.S. Department of Agriculture. Kearneysville, WV 25430.

Journal of Bacteriology, May 2007, p. 3479-3488, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.00619-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 2007 by the American Society for Microbiology. All rights reserved.