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The Escherichia coli Regulator of Sigma 70 Protein, Rsd, Can Up-Regulate Some Stress-Dependent Promoters by Sequestering Sigma 70

School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom,¹ Nara Institute of Science and Technology, Nara 630-0101, Japan,² The Rockefeller University, 1230 York Avenue, New York, New York 10021,³ Institut Pasteur, 25 rue du Dr Roux, F75724 Paris cedex 15, France⁴

Received 4 January 2007/ Accepted 19 February 2007

The Escherichia coli Rsd protein forms complexes with the RNA polymerase ⁷⁰ factor, but its biological role is not understood. Transcriptome analysis shows that overexpression of Rsd causes increased expression from some promoters whose expression depends on the alternative ³⁸ factor, and this was confirmed by experiments with lac fusions at selected promoters. The LP18 substitution in Rsd increases the Rsd-dependent stimulation of these promoter-lac fusions. Analysis with a bacterial two-hybrid system shows that the LP18 substitution in Rsd increases its interaction with ⁷⁰. Our experiments support a model in which the role of Rsd is primarily to sequester ⁷⁰, thereby increasing the levels of RNA polymerase containing the alternative ³⁸ factor.

Published ahead of print on 9 March 2007.

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