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Journal of Bacteriology, May 2007, p. 3645-3649, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.01920-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

In Vitro Analysis of the Two-Component System MtrB-MtrA from Corynebacterium glutamicum{triangledown} ,{dagger}

Nina Möker,1 Jens Krämer,2 Gottfried Unden,2 Reinhard Krämer,1 and Susanne Morbach1*

Institut für Biochemie der Universität zu Köln, Zülpicher Str. 47, 50674 Köln, Germany,1 Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität Mainz, Becherweg 15, 55099 Mainz, Germany2

Received 20 December 2006/ Accepted 2 February 2007

The two-component system MtrBA is involved in the osmostress response of Corynebacterium glutamicum. MtrB was reconstituted in a functionally active form in liposomes and showed autophosphorylation and phosphatase activity. In proteoliposomes, MtrB activity was stimulated by monovalent cations used by many osmosensors for the detection of hypertonicity. Although MtrB was activated by monovalent cations, they lead in vitro to a general stabilization of histidine kinases and do not represent the stimulus for MtrB to sense hyperosmotic stress.

* Corresponding author. Mailing address: Institut für Biochemie der Universität zu Köln, Zülpicher Str. 47, D-50674 Köln, Germany. Phone: 49-221-4706464. Fax: 49-221-470 5091. E-mail: s.morbach{at}uni-koeln.de

{triangledown} Published ahead of print on 9 February 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, May 2007, p. 3645-3649, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.01920-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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