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Journal of Bacteriology, May 2007, p. 3655-3659, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.00040-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Riboswitch Regulates Expression of the Coenzyme B12-Independent Methionine Synthase in Mycobacterium tuberculosis: Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551{triangledown} ,{dagger}

Digby F. Warner,* Suzana Savvi, Valerie Mizrahi, and Stephanie S. Dawes*

MRC/NHLS/WITS Molecular Mycobacteriology Research Unit and DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, School of Pathology, University of the Witwatersrand and the National Health Laboratory Service, Johannesburg, South Africa

Received 9 January 2007/ Accepted 12 February 2007

We observed vitamin B12-mediated growth inhibition of Mycobacterium tuberculosis strain CDC1551. The B12 sensitivity was mapped to a polymorphism in metH, encoding a coenzyme B12-dependent methionine synthase. Vitamin B12-resistant suppressor mutants of CDC1551 containing mutations in a B12 riboswitch upstream of the metE gene, which encodes a B12-independent methionine synthase, were isolated. Expression analysis confirmed that the B12 riboswitch is a transcriptional regulator of metE in M. tuberculosis.

* Corresponding author. Mailing address: Molecular Mycobacteriology Research Unit, National Health Laboratory Service, P.O. Box 1038, Johannesburg 2000, South Africa. Phone: 2711-4899370. Fax: 2711-4899397. E-mail for Digby F. Warner: digby.warner{at}nhls.ac.za. E-mail for Stephanie S. Dawes: stephanie.dawes{at}nhls.ac.za

{triangledown} Published ahead of print on 16 February 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, May 2007, p. 3655-3659, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.00040-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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