This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chai, Y.
Right arrow Articles by Winans, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chai, Y.
Right arrow Articles by Winans, S. C.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2007, p. 3674-3679, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.01274-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Reconstitution of the Biochemical Activities of the AttJ Repressor and the AttK, AttL, and AttM Catabolic Enzymes of Agrobacterium tumefaciens{triangledown} ,{dagger}

Yunrong Chai,{ddagger} Ching Sung Tsai, Hongbaek Cho, and Stephen C. Winans*

Department of Microbiology, Cornell University, Ithaca, New York 14853

Received 11 August 2006/ Accepted 9 February 2007

The attKLM operon encodes a lactonase (AttM) that hydrolyzes acylhomoserine lactone autoinducers, as well as two putative dehydrogenases (AttK and AttL). Here we show that AttK, AttL, and AttM collectively covert gamma-butyrolactone to succinate. Two metabolic intermediates, gamma-hydroxybutyrate and succinic semialdehyde, inactivated the AttJ repressor in vitro and induced attKLM transcription in vivo.

* Corresponding author. Mailing address: Department of Microbiology, 361A Wing Hall, Cornell University, Ithaca, NY 14853. Phone: (607) 255-2413. Fax: (607) 255-3904. E-mail: scw2{at}cornell.edu

{triangledown} Published ahead of print on 16 February 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138.

Journal of Bacteriology, May 2007, p. 3674-3679, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.01274-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Prell, J., Bourdes, A., Karunakaran, R., Lopez-Gomez, M., Poole, P. (2009). Pathway of {gamma}-Aminobutyrate Metabolism in Rhizobium leguminosarum 3841 and Its Role in Symbiosis. J. Bacteriol. 191: 2177-2186 [Abstract] [Full Text]  
  • Khan, S. R., Farrand, S. K. (2009). The BlcC (AttM) Lactonase of Agrobacterium tumefaciens Does Not Quench the Quorum-Sensing System That Regulates Ti Plasmid Conjugative Transfer. J. Bacteriol. 191: 1320-1329 [Abstract] [Full Text]  
  • White, C. E., Finan, T. M. (2009). Quorum Quenching in Agrobacterium tumefaciens: Chance or Necessity?. J. Bacteriol. 191: 1123-1125 [Full Text]  
  • Shepherd, R. W., Lindow, S. E. (2009). Two Dissimilar N-Acyl-Homoserine Lactone Acylases of Pseudomonas syringae Influence Colony and Biofilm Morphology. Appl. Environ. Microbiol. 75: 45-53 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»