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Reconstitution of the Biochemical Activities of the AttJ Repressor and the AttK, AttL, and AttM Catabolic Enzymes of Agrobacterium tumefaciens ^,

Yunrong Chai, Ching Sung Tsai, Hongbaek Cho, and Stephen C. Winans^*

Department of Microbiology, Cornell University, Ithaca, New York 14853

Received 11 August 2006/ Accepted 9 February 2007

The attKLM operon encodes a lactonase (AttM) that hydrolyzes acylhomoserine lactone autoinducers, as well as two putative dehydrogenases (AttK and AttL). Here we show that AttK, AttL, and AttM collectively covert gamma-butyrolactone to succinate. Two metabolic intermediates, gamma-hydroxybutyrate and succinic semialdehyde, inactivated the AttJ repressor in vitro and induced attKLM transcription in vivo.

Published ahead of print on 16 February 2007.

Supplemental material for this article may be found at http://jb.asm.org/.

Present address: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138.