This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baba, T. Articles by Hiramatsu, K. Search for Related Content PubMed PubMed Citation Articles by Baba, T. Articles by Hiramatsu, K. Pubmed/NCBI databases Gene Genome Protein

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2008, p. 300-310, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.01000-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genome Sequence of Staphylococcus aureus Strain Newman and Comparative Analysis of Staphylococcal Genomes: Polymorphism and Evolution of Two Major Pathogenicity Islands

Tadashi Baba,^1* Taeok Bae,² Olaf Schneewind,³ Fumihiko Takeuchi,⁴ and Keiichi Hiramatsu¹

Department of Microbiology and Infection Control Science, Juntendo University, Tokyo 113-8421, Japan,¹ Microbiology and Immunology, Indiana University School of Medicine, Northwest 3400 Broadway, Med. Ed. 3056, Gary, Indiana 46408,² Department of Microbiology, The University of Chicago, 920 E. 58th Street, Chicago, Illinois 60637,³ Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, United Kingdom⁴

Received 22 June 2007/ Accepted 9 October 2007

Strains of Staphylococcus aureus, an important human pathogen, display up to 20% variability in their genome sequence, and most sequence information is available for human clinical isolates that have not been subjected to genetic analysis of virulence attributes. S. aureus strain Newman, which was also isolated from a human infection, displays robust virulence properties in animal models of disease and has already been extensively analyzed for its molecular traits of staphylococcal pathogenesis. We report here the complete genome sequence of S. aureus Newman, which carries four integrated prophages, as well as two large pathogenicity islands. In agreement with the view that S. aureus Newman prophages contribute important properties to pathogenesis, fewer virulence factors are found outside of the prophages than for the highly virulent strain MW2. The absence of drug resistance genes reflects the general antibiotic-susceptible phenotype of S. aureus Newman. Phylogenetic analyses reveal clonal relationships between the staphylococcal strains Newman, COL, NCTC8325, and USA300 and a greater evolutionary distance to strains MRSA252, MW2, MSSA476, N315, Mu50, JH1, JH9, and RF122. However, polymorphism analysis of two large pathogenicity islands distributed among these strains shows that the two islands were acquired independently from the evolutionary pathway of the chromosomal backbones of staphylococcal genomes. Prophages and pathogenicity islands play central roles in S. aureus virulence and evolution.

---

* Corresponding author. Mailing address: Department of Microbiology and Infection Control Science, Juntendo University, Tokyo 113-8421, Japan. Phone: (81) 3-5802-1041. Fax: (81) 3-5684-7830. E-mail: tbaba{at}med.juntendo.ac.jp

Published ahead of print on 19 October 2007.

---

Journal of Bacteriology, January 2008, p. 300-310, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.01000-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Geissmann, T., Chevalier, C., Cros, M.-J., Boisset, S., Fechter, P., Noirot, C., Schrenzel, J., Francois, P., Vandenesch, F., Gaspin, C., Romby, P. (2009). A search for small noncoding RNAs in Staphylococcus aureus reveals a conserved sequence motif for regulation. Nucleic Acids Res 0: gkp668v1-gkp668 [Abstract] [Full Text]  
• Ng, J. W. S., Holt, D. C., Lilliebridge, R. A., Stephens, A. J., Huygens, F., Tong, S. Y. C., Currie, B. J., Giffard, P. M. (2009). Phylogenetically Distinct Staphylococcus aureus Lineage Prevalent among Indigenous Communities in Northern Australia. J. Clin. Microbiol. 47: 2295-2300 [Abstract] [Full Text]  
• Lee, D.-S., Burd, H., Liu, J., Almaas, E., Wiest, O., Barabasi, A.-L., Oltvai, Z. N., Kapatral, V. (2009). Comparative Genome-Scale Metabolic Reconstruction and Flux Balance Analysis of Multiple Staphylococcus aureus Genomes Identify Novel Antimicrobial Drug Targets. J. Bacteriol. 191: 4015-4024 [Abstract] [Full Text]  
• Somerville, G. A., Proctor, R. A. (2009). At the Crossroads of Bacterial Metabolism and Virulence Factor Synthesis in Staphylococci. Microbiol. Mol. Biol. Rev. 73: 233-248 [Abstract] [Full Text]  
• Pohl, K., Francois, P., Stenz, L., Schlink, F., Geiger, T., Herbert, S., Goerke, C., Schrenzel, J., Wolz, C. (2009). CodY in Staphylococcus aureus: a Regulatory Link between Metabolism and Virulence Gene Expression. J. Bacteriol. 191: 2953-2963 [Abstract] [Full Text]  
• Baba, T., Kuwahara-Arai, K., Uchiyama, I., Takeuchi, F., Ito, T., Hiramatsu, K. (2009). Complete Genome Sequence of Macrococcus caseolyticus Strain JSCS5402, Reflecting the Ancestral Genome of the Human-Pathogenic Staphylococci. J. Bacteriol. 191: 1180-1190 [Abstract] [Full Text]  
• Tsuru, T., Kobayashi, I. (2008). Multiple Genome Comparison within a Bacterial Species Reveals a Unit of Evolution Spanning Two Adjacent Genes in a Tandem Paralog Cluster. Mol Biol Evol 25: 2457-2473 [Abstract] [Full Text]  
• Stauff, D. L., Bagaley, D., Torres, V. J., Joyce, R., Anderson, K. L., Kuechenmeister, L., Dunman, P. M., Skaar, E. P. (2008). Staphylococcus aureus HrtA Is an ATPase Required for Protection against Heme Toxicity and Prevention of a Transcriptional Heme Stress Response. J. Bacteriol. 190: 3588-3596 [Abstract] [Full Text]