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Journal of Bacteriology, June 2008, p. 3930-3939, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.00108-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Defining the Growth Conditions and Promoter-Proximal DNA Sequences Required for Activation of Gene Expression by CreBC in Escherichia coli{triangledown}

S. James L. Cariss, Amy E. Tayler, and Matthew B. Avison*

Department of Cellular & Molecular Medicine, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom

Received 22 January 2008/ Accepted 17 March 2008

CreBC is a two-component system that controls the expression of a number of genes in Escherichia coli (called the cre regulon) that encode diverse functions, including intermediary metabolic enzymes. Using a reporter construct, we have shown that cre regulon gene expression is activated during growth in minimal media when glycolytic carbon sources are being fermented. It also is activated during aerobic growth when fermentation products are being used as carbon sources. CreB and CreC are essential for the activation of cre regulon gene expression, but CreA and CreD, encoded as part of the creABCD gene cluster, are not. CreB binds to a TTCACnnnnnnTTCAC direct repeat (the cre tag) in vitro, and this sequence, which is associated with cre regulon gene promoters, is required for the control of gene expression in vivo. These observations support the hypothesis that CreBC is a functional two-component system involved in the metabolic control of transcription in E. coli and confirm that CreB is a DNA binding transcriptional regulator.

* Corresponding author. Mailing address: Department of Cellular & Molecular Medicine, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, United Kingdom. Phone: (44) 117 33 12036. Fax: (44) 117 928 8796. E-mail: matthewb.avison{at}bristol.ac.uk

{triangledown} Published ahead of print on 28 March 2008.

Journal of Bacteriology, June 2008, p. 3930-3939, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.00108-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nikel, P. I., Zhu, J., San, K.-Y., Mendez, B. S., Bennett, G. N. (2009). Metabolic Flux Analysis of Escherichia coli creB and arcA Mutants Reveals Shared Control of Carbon Catabolism under Microaerobic Growth Conditions. J. Bacteriol. 191: 5538-5548 [Abstract] [Full Text]  


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