This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tran-Nguyen, L. T. T.
Right arrow Articles by Gibb, K. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tran-Nguyen, L. T. T.
Right arrow Articles by Gibb, K. S.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2008, p. 3979-3991, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.01301-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparative Genome Analysis of "Candidatus Phytoplasma australiense" (Subgroup tuf-Australia I; rp-A) and "Ca. Phytoplasma asteris" Strains OY-M and AY-WB{triangledown} ,{dagger}

L. T. T. Tran-Nguyen,*1,{ddagger} M. Kube,2 B. Schneider,3 R. Reinhardt,2 and K. S. Gibb1

Charles Darwin University, School of Environmental and Life Sciences, Darwin, NT, 0909, Australia,1 Max Planck Institute for Molecular Genetics, Berlin, Germany,2 Julius Kuehn Institute, Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Dossenheim, Germany3

Received 10 August 2007/ Accepted 12 March 2008

The chromosome sequence of "Candidatus Phytoplasma australiense" (subgroup tuf-Australia I; rp-A), associated with dieback in papaya, Australian grapevine yellows in grapevine, and several other important plant diseases, was determined. The circular chromosome is represented by 879,324 nucleotides, a GC content of 27%, and 839 protein-coding genes. Five hundred two of these protein-coding genes were functionally assigned, while 337 genes were hypothetical proteins with unknown function. Potential mobile units (PMUs) containing clusters of DNA repeats comprised 12.1% of the genome. These PMUs encoded genes involved in DNA replication, repair, and recombination; nucleotide transport and metabolism; translation; and ribosomal structure. Elements with similarities to phage integrases found in these mobile units were difficult to classify, as they were similar to both insertion sequences and bacteriophages. Comparative analysis of "Ca. Phytoplasma australiense" with "Ca. Phytoplasma asteris" strains OY-M and AY-WB showed that the gene order was more conserved between the closely related "Ca. Phytoplasma asteris" strains than to "Ca. Phytoplasma australiense." Differences observed between "Ca. Phytoplasma australiense" and "Ca. Phytoplasma asteris" strains included the chromosome size (18,693 bp larger than OY-M), a larger number of genes with assigned function, and hypothetical proteins with unknown function.

* Corresponding author. Present address: Department of Primary Industry, Fisheries and Mines, Darwin, NT 0801, Australia. Phone: 61 8 8999 2235. Fax: 61 8 8999 2312. E-mail: lucy.tran-nguyen{at}nt.gov.au

{triangledown} Published ahead of print on 21 March 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, June 2008, p. 3979-3991, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.01301-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhao, Y., Wei, W., Lee, I.-M., Shao, J., Suo, X., Davis, R. E. (2009). Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int. J. Syst. Evol. Microbiol. 59: 2582-2593 [Abstract] [Full Text]  
  • Cimerman, A., Pacifico, D., Salar, P., Marzachi, C., Foissac, X. (2009). Striking Diversity of vmp1, a Variable Gene Encoding a Putative Membrane Protein of the Stolbur Phytoplasma. Appl. Environ. Microbiol. 75: 2951-2957 [Abstract] [Full Text]  
  • Hoshi, A., Oshima, K., Kakizawa, S., Ishii, Y., Ozeki, J., Hashimoto, M., Komatsu, K., Kagiwada, S., Yamaji, Y., Namba, S. (2009). A unique virulence factor for proliferation and dwarfism in plants identified from a phytopathogenic bacterium. Proc. Natl. Acad. Sci. USA 106: 6416-6421 [Abstract] [Full Text]  
  • Wei, W., Davis, R. E., Jomantiene, R., Zhao, Y. (2008). Ancient, recurrent phage attacks and recombination shaped dynamic sequence-variable mosaics at the root of phytoplasma genome evolution. Proc. Natl. Acad. Sci. USA 105: 11827-11832 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»