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Journal of Bacteriology, June 2008, p. 4061-4068, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.01954-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

CabC, an EF-Hand Calcium-Binding Protein, Is Involved in Ca2+-Mediated Regulation of Spore Germination and Aerial Hypha Formation in Streptomyces coelicolor{triangledown}

Sheng-Lan Wang, Ke-Qiang Fan, Xu Yang, Zeng-Xi Lin, Xin-Ping Xu, and Ke-Qian Yang*

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China

Received 16 December 2007/ Accepted 14 March 2008

Ca2+ was reported to regulate spore germination and aerial hypha formation in streptomycetes; the underlying mechanism of this regulation is not known. cabC, a gene encoding an EF-hand calcium-binding protein, was disrupted or overexpressed in Streptomyces coelicolor M145. On R5– agar, the disruption of cabC resulted in denser aerial hyphae with more short branches, swollen hyphal tips, and early-germinating spores on the spore chain, while cabC overexpression significantly delayed development. Manipulation of the Ca2+ concentration in R5– agar could reverse the phenotypes of cabC disruption or overexpression mutants and mimic mutant phenotypes with M145, suggesting that the mutant phenotypes were due to changes in the intracellular Ca2+ concentration. CabC expression was strongly activated in aerial hyphae, as determined by Western blotting against CabC and confocal laser scanning microscopy detection of CabC::enhanced green fluorescent protein (EGFP). CabC::EGFP fusion proteins were evenly distributed in substrate mycelia, aerial mycelia, and spores. Taken together, these results demonstrate that CabC is involved in Ca2+-mediated regulation of spore germination and aerial hypha formation in S. coelicolor. CabC most likely acts as a Ca2+ buffer and exerts its regulatory effects by controlling the intracellular Ca2+ concentration.

* Corresponding author. Mailing address: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. Phone: 86-10-64807457. Fax: 86-10-64807459. E-mail: yangkq{at}im.ac.cn

{triangledown} Published ahead of print on 28 March 2008.

Journal of Bacteriology, June 2008, p. 4061-4068, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.01954-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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