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Journal of Bacteriology, July 2008, p. 4541-4548, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.00249-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cortex Peptidoglycan Lytic Activity in Germinating Bacillus anthracis Spores

Melissa M. Dowd, Benjamin Orsburn, and David L. Popham^*

Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia 24061

Received 19 February 2008/ Accepted 22 April 2008

Bacterial endospore dormancy and resistance properties depend on the relative dehydration of the spore core, which is maintained by the spore membrane and its surrounding cortex peptidoglycan wall. During spore germination, the cortex peptidoglycan is rapidly hydrolyzed by lytic enzymes packaged into the dormant spore. The peptidoglycan structures in both dormant and germinating Bacillus anthracis Sterne spores were analyzed. The B. anthracis dormant spore peptidoglycan was similar to that found in other species. During germination, B. anthracis released peptidoglycan fragments into the surrounding medium more quickly than some other species. A major lytic enzymatic activity was a glucosaminidase, probably YaaH, that cleaved between N-acetylglucosamine and muramic--lactam. An epimerase activity previously proposed to function on spore peptidoglycan was not detected, and it is proposed that glucosaminidase products were previously misidentified as epimerase products. Spore cortex lytic enzymes and their regulators are attractive targets for development of germination inhibitors to kill spores and for development of activators to cause loss of resistance properties for decontamination of spore release sites.

Published ahead of print on 2 May 2008.

Present address: Department of Epidemiology, Rollins School of Public Health, Emory University, Atlanta, GA 30333.