This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by May, K. L.
Right arrow Articles by Morona, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by May, K. L.
Right arrow Articles by Morona, R.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2008, p. 4666-4676, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.00093-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Mutagenesis of the Shigella flexneri Autotransporter IcsA Reveals Novel Functional Regions Involved in IcsA Biogenesis and Recruitment of Host Neural Wiscott-Aldrich Syndrome Protein{triangledown} ,{dagger}

Kerrie L. May and Renato Morona*

Australian Bacterial Pathogenesis Program, Discipline of Microbiology and Immunology, School of Molecular and Biomedical Science, University of Adelaide, South Australia, Australia

Received 17 January 2008/ Accepted 28 April 2008

The IcsA (VirG) protein of Shigella flexneri is a polarly localized, outer membrane protein that is essential for virulence. Within host cells, IcsA activates the host actin regulatory protein, neural Wiskott-Aldrich syndrome protein (N-WASP), which in turn recruits the Arp2/3 complex, which nucleates host actin to form F-actin comet tails and initiate bacterial motility. Linker insertion mutagenesis was undertaken to randomly introduce 5-amino-acid in-frame insertions within IcsA. Forty-seven linker insertion mutants were isolated and expressed in S. flexneri {Delta}icsA strains. Mutants were characterized for IcsA protein production, cell surface expression and localization, intercellular spreading, F-actin comet tail formation, and N-WASP recruitment. Using this approach, we have identified a putative autochaperone region required for IcsA biogenesis, and our data suggest an additional region, not previously identified, is required for N-WASP recruitment.

* Corresponding author. Mailing address: School of Molecular and Biomedical Science, University of Adelaide, South Australia 5005, Australia. Phone: 61-8-83034151. Fax: 61-8-83037532. E-mail: renato.morona{at}adelaide.edu.au

{triangledown} Published ahead of print on 2 May 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, July 2008, p. 4666-4676, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.00093-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»