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Journal of Bacteriology, July 2008, p. 4759-4763, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.00399-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Release of Small Molecules during Germination of Spores of Bacillus Species{triangledown}

Barbara Setlow, Paul G. Wahome, and Peter Setlow*

Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030-3305

Received 20 March 2008/ Accepted 26 April 2008

Free amino acids, dipicolinic acid, and unidentified small molecules were released early in Bacillus spore germination before hydrolysis of the peptidoglycan cortex, but adenine nucleotides and 3-phosphoglycerate were not. These results indicate that early in germination there is a major selective change in the permeability of the spore's inner membrane.


* Corresponding author. Mailing address: Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030-3305. Phone: (860) 679-2607. Fax: (860) 679-3408. E-mail: setlow{at}nso2.uchc.edu

{triangledown} Published ahead of print on 9 May 2008.


Journal of Bacteriology, July 2008, p. 4759-4763, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.00399-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.




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