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Journal of Bacteriology, July 2008, p. 4971-4978, Vol. 190, No. 14
0021-9193/08/$08.00+0     doi:10.1128/JB.01849-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Cellulose Synthase-Like Protein Involved in Hyphal Tip Growth and Morphological Differentiation in Streptomyces{triangledown}

Hongbin Xu,1 Keith F. Chater,2 Zixin Deng,3 and Meifeng Tao1*

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China,1 John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom,2 Laboratory of Microbial Metabolism, Shanghai Jiaotong University, Shanghai 200030, China3

Received 23 November 2007/ Accepted 3 May 2008

Cellulose synthase and cellulose synthase-like proteins, responsible for synthesizing β-glucan-containing polysaccharides, play a fundamental role in cellular architectures, such as plant cell and tissue morphogenesis, bacterial biofilm formation, and fruiting-body development. However, the roles of the proteins involved in the developmental process are not well understood. Here, we report that a cellulose synthase-like protein (CslASc) in Streptomyces has a function in hyphal tip growth and morphological differentiation. The cslASc replacement mutant showed pleiotropic defects, including the severe delay of aerial-hyphal formation and altered cell wall morphology. Calcofluor white fluorescence analysis demonstrated that polysaccharide synthesis at hyphal tips was dependent on CslASc. cslASc was constitutively transcribed, and an enhanced green fluorescent protein-CslASc fusion protein was mostly located at the hyphal tips. An extract enriched in morphogenetic chaplin proteins promoted formation of aerial hyphae by the mutant. Furthermore, a two-hybrid experiment indicated that the glycosyltransferase domain of CslASc interacted with the tropomyosin-like polarity-determining DivIVA protein, suggesting that the tip-located DivIVA governed tip recruitment of the CslASc membrane protein. These results imply that the cellulose synthase-like protein couples extracellular and cytoskeletal components functioning in tip growth and cell development.

* Corresponding author. Mailing address: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Phone: 86 027 87283702. Fax: 86 027 87280670. E-mail: tao_meifeng{at}yahoo.com

{triangledown} Published ahead of print on 16 May 2008.

Journal of Bacteriology, July 2008, p. 4971-4978, Vol. 190, No. 14
0021-9193/08/$08.00+0     doi:10.1128/JB.01849-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hempel, A. M., Wang, S.-b., Letek, M., Gil, J. A., Flardh, K. (2008). Assemblies of DivIVA Mark Sites for Hyphal Branching and Can Establish New Zones of Cell Wall Growth in Streptomyces coelicolor. J. Bacteriol. 190: 7579-7583 [Abstract] [Full Text]  


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