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Journal of Bacteriology, July 2008, p. 5127-5131, Vol. 190, No. 14
0021-9193/08/$08.00+0     doi:10.1128/JB.00395-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The c-Type Cytochrome OmcA Localizes to the Outer Membrane upon Heterologous Expression in Escherichia coli

Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom,¹ School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom,² Division of Molecular and Environmental Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom³

Received 20 March 2008/ Accepted 9 May 2008

We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.

Published ahead of print on 16 May 2008.