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Journal of Bacteriology, August 2008, p. 5217-5223, Vol. 190, No. 15
0021-9193/08/$08.00+0     doi:10.1128/JB.00243-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Plasmid-Borne Shewanella algae Gene, qnrA3, and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae{triangledown}

Christine Lascols,1 Isabelle Podglajen,2,3,4,5 Charlotte Verdet,6 Valérie Gautier,6 Laurent Gutmann,2,3,4,5 Claude-James Soussy,1,7 Ekkehard Collatz,3 and Emmanuelle Cambau1,7*

Université Paris 12, IFR10, Bactériologie, F-94000, Creteil, France,1 AP-HP, Hôpital Européen Georges Pompidou, Service de Microbiologie, Paris 15, F-75015, France,2 INSERM UMR 872, Centre de Recherche des Cordeliers, Pôle 4-Equipe 12, F-75006, France,3 Université Pierre et Marie Curie, UMR S 872, Paris 6, F-75015, France,4 Université Paris-Descartes, UMR S 872, Paris 5, F-75005, France,5 Université Pierre et Marie Curie, UPRES EA 2392, Paris 6, F-75006 France,6 AP-HP, Hôpital Henri Mondor, Service de Bactériologie-Virologie-Hygiène, Créteil, F-94000, France7

Received 18 February 2008/ Accepted 7 May 2008

The plasmid-borne quinolone resistance gene qnrA1 is prevalent in multidrug-resistant Enterobacteriaceae. A chromosomally encoded homologue in Shewanella algae, qnrA3, has been described. We isolated two qnrA3-positive strains, one of Klebsiella pneumoniae (He96) and one of Kluyvera ascorbata (Kas96), from the feces of an immunocompromised outpatient. The qnrA3 allele was identical to that of S. algae except for 5 nucleotides and differed from qnrA1 by 29 nucleotides affecting three amino acids. The analysis of the qnrA3 genetic environment showed that qnrA3 was inserted downstream from an ISCR1 element at a recombination crossover site described for other resistance genes, including qnrA1, and immediately upstream from IS26, a situation not described before. IS26 preceded an incomplete class 1 integron which contained, among other genes, aac(6')-Ib-cr, another transferable quinolone resistance gene, and the β-lactamase gene blaOXA-1/30. The 10-kb fragment encompassing qnrA3 was compared to previously described qnrA1-containing plasmids and multidrug-resistant plasmids; it shares identical sequences with pC15a, pHSH2, pQR1, pQKp311H, and pSAL-1 but with rearrangements, deletions, and mutations. Conjugal transfer of qnrA3 was highly efficient (10–2) from K. pneumoniae He96 or K. ascorbata Kas96 to Escherichia coli J53 but less so (10–5) from either donor to a clinical strain of Enterobacter cloacae. This first description of a plasmid-borne copy and of the in vitro transfer of qnrA3 is taken to illustrate its likely in vivo transfer from S. algae to the Enterobacteriaceae.

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Virologie-Hygiène, Centre Hospitalier Universitaire Henri Mondor, 51 avenue de Lattre de Tassigny, 94010 Créteil Cedex, France. Phone: 33 1 49 81 28 31. Fax: 33 1 49 81 28 39. E-mail: emmanuelle.cambau{at}hmn.aphp.fr

{triangledown} Published ahead of print on 30 May 2008.

Journal of Bacteriology, August 2008, p. 5217-5223, Vol. 190, No. 15
0021-9193/08/$08.00+0     doi:10.1128/JB.00243-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Carattoli, A. (2009). Resistance Plasmid Families in Enterobacteriaceae. Antimicrob. Agents Chemother. 53: 2227-2238 [Full Text]  
  • Wang, M., Guo, Q., Xu, X., Wang, X., Ye, X., Wu, S., Hooper, D. C., Wang, M. (2009). New Plasmid-Mediated Quinolone Resistance Gene, qnrC, Found in a Clinical Isolate of Proteus mirabilis. Antimicrob. Agents Chemother. 53: 1892-1897 [Abstract] [Full Text]  


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