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Journal of Bacteriology, August 2008, p. 5230-5238, Vol. 190, No. 15
0021-9193/08/$08.00+0     doi:10.1128/JB.00194-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

NagA-Dependent Uptake of N-Acetyl-Glucosamine and N-Acetyl-Chitin Oligosaccharides across the Outer Membrane of Caulobacter crescentus

Simone Eisenbeis,¹ Stefanie Lohmiller,¹ Marianne Valdebenito,¹ Stefan Leicht,² and Volkmar Braun^1,3*

Microbiology/Membrane Physiology,¹ Proteome Center, University of Tübingen,² Max Planck Institute for Developmental Biology, Department of Protein Evolution, Tübingen, Germany³

Received 7 February 2008/ Accepted 22 May 2008

Among the 67 predicted TonB-dependent outer membrane transporters of Caulobacter crescentus, NagA was found to be essential for growth on N-acetyl-β-D-glucosamine (GlcNAc) and larger chitin oligosaccharides. NagA (93 kDa) has a predicted typical domain structure of an outer membrane transport protein: a signal sequence, the TonB box EQVVIT, a hatch domain of 147 residues, and a β-barrel composed of 22 antiparallel β-strands linked by large surface loops and very short periplasmic turns. Mutations in tonB1 and exbBD, known to be required for maltose transport via MalA in C. crescentus, and in two additional predicted tonB genes (open reading frames cc2327 and cc3508) did not affect NagA-mediated GlcNAc uptake. nagA is located in a gene cluster that encodes a predicted PTS sugar transport system and two enzymes that convert GlcNAc-6-P to fructose-6-P. Since a nagA insertion mutant did not grow on and transport GlcNAc, diffusion of GlcNAc through unspecific porins in the outer membrane is excluded. Uptake of GlcNAc into tonB and exbBD mutants and reduction but not abolishment of GlcNAc transport by agents which dissipate the electrochemical potential of the cytoplasmic membrane (0.1 mM carbonyl cyanide 3-chlorophenylhydrazone and 1 mM 2,4-dinitrophenol) suggest diffusion of GlcNAc through a permanently open pore of NagA. Growth on (GlcNAc)₃ and (GlcNAc)₅ requires ExbB and ExbD, indicating energy-coupled transport by NagA. We propose that NagA forms a small pore through which GlcNAc specifically diffuses into the periplasm and functions as an energy-coupled transporter for the larger chitin oligosaccharides.

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* Corresponding author. Mailing address: Max Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstrasse 35, 72076 Tübingen, Germany. Phone: (49) 7071 601343. Fax: (49) 7071 601300. E-mail: volkmar.braun{at}tuebingen.mpg.de

Published ahead of print on 6 June 2008.

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Journal of Bacteriology, August 2008, p. 5230-5238, Vol. 190, No. 15
0021-9193/08/$08.00+0     doi:10.1128/JB.00194-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

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