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Journal of Bacteriology, August 2008, p. 5362-5367, Vol. 190, No. 15
0021-9193/08/$08.00+0     doi:10.1128/JB.00330-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Cell Cycle Characteristics of Crenarchaeota: Unity among Diversity{triangledown}

Magnus Lundgren,1,{dagger}* Laurence Malandrin,1,{dagger},{ddagger} Stefan Eriksson,1 Harald Huber,2 and Rolf Bernander1

Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University, Norbyvägen 18C, SE-752 36 Uppsala, Sweden,1 Department of Microbiology, University of Regensburg, Universitätsstraße 31, D-93053 Regensburg, Germany2

Received 5 March 2008/ Accepted 16 May 2008

The hyperthermophilic archaea Acidianus hospitalis, Aeropyrum pernix, Pyrobaculum aerophilum, Pyrobaculum calidifontis, and Sulfolobus tokodaii representing three different orders in the phylum Crenarchaeota were analyzed by flow cytometry and combined phase-contrast and epifluorescence microscopy. The overall organization of the cell cycle was found to be similar in all species, with a short prereplicative period and a dominant postreplicative period that accounted for 64 to 77% of the generation time. Thus, in all Crenarchaeota analyzed to date, cell division and initiation of chromosome replication occur in close succession, and a long time interval separates termination of replication from cell division. In Pyrobaculum, chromosome segregation overlapped with or closely followed DNA replication, and further genome separation appeared to occur concomitant with cellular growth. Cell division in P. aerophilum took place without visible constriction.

* Corresponding author. Present address: Laboratory of Microbiology, Agrotechnology and Food Sciences Group, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, Netherlands. Phone: 31-317482105. Fax: 31-317483829. E-mail: magnus.lundgren{at}wur.nl

{triangledown} Published ahead of print on 23 May 2008.

{dagger} M.L. and L.M. contributed equally to this work.

{ddagger} Present address: INRA, Ecole Nationale Veterinaire de Nantes UMR1300, Bio-agression, Epidémiologie et Analyse de Risques, BP 40706, F-44307 Nantes, France.

Journal of Bacteriology, August 2008, p. 5362-5367, Vol. 190, No. 15
0021-9193/08/$08.00+0     doi:10.1128/JB.00330-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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