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Journal of Bacteriology, August 2008, p. 5404-5411, Vol. 190, No. 15
0021-9193/08/$08.00+0     doi:10.1128/JB.00415-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Effects of pH and Temperature on the Composition of Polar Lipids in Thermoplasma acidophilum HO-62 {triangledown} ,{dagger}

Haruo Shimada,1 Naoki Nemoto,1 Yasuo Shida,2 Tairo Oshima,1 and Akihiko Yamagishi1*

Department of Molecular Biology, Tokyo University of Pharmacy and Life Science,1 Department of Chemical Analysis, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan2

Received 25 March 2008/ Accepted 26 May 2008

Thermoplasma acidophilum HO-62 was grown at different pHs and temperatures, and its polar lipid compositions were determined. Although the number of cyclopentane rings in the caldarchaeol moiety increased when T. acidophilum was cultured at high temperature, the number decreased at low pHs. Glycolipids, phosphoglycolipids, and phospholipids were analyzed by high-performance liquid chromatography with an evaporative light-scattering detector. The amount of caldarchaeol with more than two sugar units on one side increased under low-pH and high-temperature conditions. The amounts of glycolipids increased and those of phosphoglycolipids decreased under these conditions. The proton permeability of the liposomes obtained from the phosphoglycolipids that contained two or more sugar units was lower than that of the liposomes obtained from the phosphoglycolipids that contained one sugar unit. From these results, we propose the hypothesis that T. acidophilum adapts to low pHs and high temperatures by extending sugar chains on their cell surfaces, as well as by varying the number of cyclopentane rings.

* Corresponding author. Mailing address: Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Phone: 81 426 76 7139. Fax: 81 426 76 7145. E-mail: yamagish{at}LS.toyaku.ac.jp

{triangledown} Published ahead of print on 6 June 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, August 2008, p. 5404-5411, Vol. 190, No. 15
0021-9193/08/$08.00+0     doi:10.1128/JB.00415-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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