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Journal of Bacteriology, August 2008, p. 5720-5728, Vol. 190, No. 16
0021-9193/08/$08.00+0     doi:10.1128/JB.00455-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Autoregulation of swrAA and Motility in Bacillus subtilis{triangledown}

Cinzia Calvio,* Cecilia Osera, Giuseppe Amati, and Alessandro Galizzi

Dipartimento di Genetica e Microbiologia A. Buzzati-Traverso, Università degli Studi di Pavia, 27100 Pavia, Italy

Received 2 April 2008/ Accepted 9 June 2008

We demonstrate that transcription of the gene swrAA, required for swarming migration in Bacillus subtilis, is driven by two promoters: a sigD-dependent promoter and a putative sigA-dependent promoter, which is inactive during growth in liquid Luria-Bertani medium and becomes active in the presence of the phosphorylated form of the response regulator DegU or on semisolid surfaces. Since sigD transcription is enhanced by SwrAA, this finding reveals that swrA expression is controlled by a positive feedback loop. We also demonstrate that the positive action of SwrAA in swimming and swarming motility is prevented in strains carrying a deletion of the two-component system degS-degU and that this effect is independent of swrAA transcription. Therefore, both DegU and SwrAA must be present to achieve full motility in B. subtilis.

* Corresponding author. Mailing address: Dipartimento di Genetica e Microbiologia Buzzati-Traverso, Università degli Studi di Pavia, Via Ferrata 1, 27100 Pavia, Italy. Phone: 39-0382-985545. Fax: 39-0382-528496. E-mail: cinzia.calvio{at}unipv.it

{triangledown} Published ahead of print on 20 June 2008.

Journal of Bacteriology, August 2008, p. 5720-5728, Vol. 190, No. 16
0021-9193/08/$08.00+0     doi:10.1128/JB.00455-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Osera, C., Amati, G., Calvio, C., Galizzi, A. (2009). SwrAA activates poly-{gamma}-glutamate synthesis in addition to swarming in Bacillus subtilis. Microbiology 155: 2282-2287 [Abstract] [Full Text]  


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