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Journal of Bacteriology, September 2008, p. 5915-5923, Vol. 190, No. 17
0021-9193/08/$08.00+0     doi:10.1128/JB.00148-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

AinS Quorum Sensing Regulates the Vibrio fischeri Acetate Switch{triangledown}

Sarah V. Studer, Mark J. Mandel, and Edward G. Ruby*

Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison, Wisconsin 53706-1521

Received 28 January 2008/ Accepted 12 May 2008

The marine bacterium Vibrio fischeri uses two acyl-homoserine lactone (acyl-HSL) quorum-sensing systems. The earlier signal, octanoyl-HSL, produced by AinS, is required for normal colonization of the squid Euprymna scolopes and, in culture, is necessary for a normal growth yield. In examining the latter requirement, we found that during growth in a glycerol/tryptone-based medium, wild-type V. fischeri cells initially excrete acetate but, in a metabolic shift termed the acetate switch, they subsequently utilize the acetate, removing it from the medium. In contrast, an ainS mutant strain grown in this medium does not remove the excreted acetate, which accumulates to lethal levels. The acetate switch is characterized by the induction of acs, the gene encoding acetyl coenzyme A (acetyl-CoA) synthetase, leading to uptake of the excreted acetate. Wild-type cells induce an acs transcriptional reporter 25-fold, coincident with the disappearance of the extracellular acetate; in contrast, the ainS mutant did not display significant induction of the acs reporter. Supplementation of the medium of an ainS mutant with octanoyl-HSL restored normal levels of acs induction and acetate uptake. Additional mutant analyses indicated that acs regulation was accomplished through the regulator LitR but was independent of the LuxIR quorum-signaling pathway. Importantly, the acs mutant of V. fischeri has a competitive defect when colonizing the squid, indicating the importance of proper control of acetate metabolism in the light of organ symbiosis. This is the first report of quorum-sensing control of the acetate switch, and it indicates a metabolic connection between acetate utilization and cell density.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, 5203 Microbial Sciences Building, Madison, WI 53706. Phone: (608) 262-5911. Fax: (608) 262-8418. E-mail: egruby{at}wisc.edu

{triangledown} Published ahead of print on 16 May 2008.

Journal of Bacteriology, September 2008, p. 5915-5923, Vol. 190, No. 17
0021-9193/08/$08.00+0     doi:10.1128/JB.00148-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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  • Lyell, N. L., Dunn, A. K., Bose, J. L., Vescovi, S. L., Stabb, E. V. (2008). Effective Mutagenesis of Vibrio fischeri by Using Hyperactive Mini-Tn5 Derivatives. Appl. Environ. Microbiol. 74: 7059-7063 [Abstract] [Full Text]  


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