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Journal of Bacteriology, September 2008, p. 5963-5971, Vol. 190, No. 17
0021-9193/08/$08.00+0     doi:10.1128/JB.00622-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evidence of Complex Transcriptional, Translational, and Posttranslational Regulation of the Extracytoplasmic Function Sigma Factor {sigma}E in Mycobacterium tuberculosis{triangledown} ,{dagger}

Valentina Donà,1 Sébastien Rodrigue,2,§ Elisa Dainese,1 Giorgio Palù,1 Luc Gaudreau,2 Riccardo Manganelli,1* and Roberta Provvedi3*

Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, Italy,1 Centre de Recherche sur les Mécanismes du Fonctionnement Cellulaire, Université de Sherbrooke, Sherbrooke, Canada J1K 2R1,2 Department of Biology, University of Padua, Padua, Italy3

Received 5 May 2008/ Accepted 24 June 2008

The extracytoplasmic factor (ECF) sigma factor {sigma}E is one of the most studied sigma factors of Mycobacterium tuberculosis. It has been shown to be involved in virulence as well as in survival under conditions of high temperature, alkaline pH, and exposure to detergents and oxidative stress. Unlike many ECF sigma factors, {sigma}E does not directly regulate the transcription of its own gene. Two promoters have been identified upstream of the sigE gene; one is regulated by the two-component system MprAB, while the other has been shown to be {sigma}H dependent. In this paper, we further characterize the regulation of {sigma}E by identifying its anti-sigma factor and a previously unknown promoter. Finally, we show that sigE can be translated from three different translational start codons, depending on the promoter used. Taken together, our data demonstrate that {sigma}E not only is subjected to complex transcriptional regulation but is also controlled at the translational and posttranslational levels.

* Corresponding author. Mailing address: Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Via Gabelli, 63 35100 Padova, Italy. Phone: 39 49 827 2366. Fax: 39 49 827 2355. E-mail for Roberta Provvedi: roberta.provvedi{at}unipd.it. E-mail for Riccardo Manganelli: riccardo.manganelli{at}unipd.it

{triangledown} Published ahead of print on 7 July 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

§ Present address: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA.

Journal of Bacteriology, September 2008, p. 5963-5971, Vol. 190, No. 17
0021-9193/08/$08.00+0     doi:10.1128/JB.00622-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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