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Journal of Bacteriology, September 2008, p. 6076-6083, Vol. 190, No. 18
0021-9193/08/$08.00+0     doi:10.1128/JB.00660-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Translational Regulation by an Intramolecular Stem-Loop Is Required for Intermolecular RNA Regulation of the par Addiction Module{triangledown} ,{dagger}

Sonia Shokeen, Smita Patel,{ddagger} Tony J. Greenfield,§ Cassandra Brinkman, and Keith E. Weaver*

Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, South Dakota 57069

Received 12 May 2008/ Accepted 10 July 2008

The par stability determinant of Enterococcus faecalis plasmid pAD1 is the only antisense RNA-regulated addiction module identified to date in gram-positive bacteria. par encodes two small, convergently transcribed RNAs, designated RNA I and RNA II, that function as the toxin (Fst)-encoding and antitoxin components, respectively. Previous work showed that structures at the 5' end of RNA I are important in regulating its translation. The work presented here reveals that a stem-loop sequestering the Fst ribosome binding site is required for translational repression but a helix sequestering the 5' end of RNA I is not. Furthermore, disruption of the stem-loop prevented RNA II-mediated repression of Fst translation in vivo. Finally, although Fst-encoding wild-type RNA I is not toxic in Escherichia coli, mutations affecting stem-loop stability resulted in toxicity in this host, presumably due to increased translation.

* Corresponding author. Mailing address: Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069. Phone: (605) 677-5169. Fax: (605) 677-6381. E-mail: kweaver{at}usd.edu

{triangledown} Published ahead of print on 18 July 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Institute for Neurodegenerative Diseases, Box 0518/SF, University of California, San Francisco, CA 94143.

§ Present address: Department of Biology, Southwest Minnesota State University, 1501 State St., Marshall, MN 56258.

Journal of Bacteriology, September 2008, p. 6076-6083, Vol. 190, No. 18
0021-9193/08/$08.00+0     doi:10.1128/JB.00660-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shokeen, S., Greenfield, T. J., Ehli, E. A., Rasmussen, J., Perrault, B. E., Weaver, K. E. (2009). An Intramolecular Upstream Helix Ensures the Stability of a Toxin-Encoding RNA in Enterococcus faecalis. J. Bacteriol. 191: 1528-1536 [Abstract] [Full Text]  


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