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Journal of Bacteriology, October 2008, p. 6458-6466, Vol. 190, No. 19
0021-9193/08/$08.00+0     doi:10.1128/JB.00780-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of the Dicarboxylate Uptake System DccT in Corynebacterium glutamicum{triangledown}

Jung-Won Youn,1,# Elena Jolkver,2,# Reinhard Krämer,2 Kay Marin,2* and Volker F. Wendisch1*

Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Muenster, Germany,1 Institute of Biochemistry, Cologne University, Cologne, Germany2

Received 3 June 2008/ Accepted 21 July 2008

Many bacteria can utilize C4-carboxylates as carbon and energy sources. However, Corynebacterium glutamicum ATCC 13032 is not able to use tricarboxylic acid cycle intermediates such as succinate, fumarate, and L-malate as sole carbon sources. Upon prolonged incubation, spontaneous mutants which had gained the ability to grow on succinate, fumarate, and L-malate could be isolated. DNA microarray analysis showed higher mRNA levels of cg0277, which subsequently was named dccT, in the mutants than in the wild type, and transcriptional fusion analysis revealed that a point mutation in the promoter region of dccT was responsible for increased expression. The overexpression of dccT was sufficient to enable the C. glutamicum wild type to grow on succinate, fumarate, and L-malate as the sole carbon sources. Biochemical analyses revealed that DccT, which is a member of the divalent anion/Na+ symporter family, catalyzes the effective uptake of dicarboxylates like succinate, fumarate, L-malate, and likely also oxaloacetate in a sodium-dependent manner.

* Corresponding author. Mailing address for Volker F. Wendisch: Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster, Corrensstr. 3, D-48149 Muenster, Germany. Phone: 49-251-833 9827. Fax: 49-251-833 8388. E-mail: wendisch{at}uni-muenster.de. Mailing address for Kay Marin: Institute of Biochemistry, Cologne University, Cologne, Germany. Phone: 49-221-470 6476. Fax: 49-221-470 5091. E-mail: kay.marin{at}uni-koeln.de

{triangledown} Published ahead of print on 25 July 2008.

# Both authors contributed equally.

Journal of Bacteriology, October 2008, p. 6458-6466, Vol. 190, No. 19
0021-9193/08/$08.00+0     doi:10.1128/JB.00780-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Youn, J.-W., Jolkver, E., Kramer, R., Marin, K., Wendisch, V. F. (2009). Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum. J. Bacteriol. 191: 5480-5488 [Abstract] [Full Text]  
  • Jolkver, E., Emer, D., Ballan, S., Kramer, R., Eikmanns, B. J., Marin, K. (2009). Identification and Characterization of a Bacterial Transport System for the Uptake of Pyruvate, Propionate, and Acetate in Corynebacterium glutamicum. J. Bacteriol. 191: 940-948 [Abstract] [Full Text]  


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