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Journal of Bacteriology, January 2008, p. 750-754, Vol. 190, No. 2
0021-9193/08/$08.00+0     doi:10.1128/JB.01116-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Protein-Protein Interactions within Type III Secretion System-Dependent Pili of Rhizobium sp. Strain NGR234{triangledown}

Maged M. Saad,{dagger} Christian Staehelin,{ddagger} William J. Broughton,* and William J. Deakin

LBMPS, Université de Genève, Sciences III, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland

Received 16 July 2007/ Accepted 25 October 2007

Pili synthesized by the type III secretion system of Rhizobium species strain NGR234 are essential for protein secretion and thus for efficient symbiosis with many legumes. Isolation and partial purification of these pili showed that they are composed of at least three proteins, NopA, NopB, and NopX. Using biochemical assays, we show here that these proteins interact directly with one another.


* Corresponding author. Mailing address: LBMPS, Université de Genève, Sciences III, 30 quai Ernest-Ansermet, 1211 Genève 4, Switzerland. Phone: 41 22 379 3108. Fax: 41 22 379 3009. E-mail: william.broughton{at}bioveg.unige.ch

{triangledown} Published ahead of print on 2 November 2007.

{dagger} Present address: LIPM, UMR CNRS-INRA 2594/441, BP 52627, 31326 Castanet Tolosan Cedex, France.

{ddagger} Present address: School of Life Sciences, SunYat-Sen (Zhongshan) University, 135 Xingangxi Road, Guangzhou, 510275, China.


Journal of Bacteriology, January 2008, p. 750-754, Vol. 190, No. 2
0021-9193/08/$08.00+0     doi:10.1128/JB.01116-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Dai, W.-J., Zeng, Y., Xie, Z.-P., Staehelin, C. (2008). Symbiosis-Promoting and Deleterious Effects of NopT, a Novel Type 3 Effector of Rhizobium sp. Strain NGR234. J. Bacteriol. 190: 5101-5110 [Abstract] [Full Text]