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Journal of Bacteriology, January 2008, p. 772-776, Vol. 190, No. 2
0021-9193/08/$08.00+0     doi:10.1128/JB.01390-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Novel Caffeine Dehydrogenase in Pseudomonas sp. Strain CBB1 Oxidizes Caffeine to Trimethyluric Acid{triangledown}

Chi Li Yu, Yogesh Kale, Sridhar Gopishetty, Tai Man Louie, and Mani Subramanian*

Center for Biocatalysis & Bioprocessing and Department of Chemical & Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242

Received 27 August 2007/ Accepted 24 October 2007

A unique heterotrimeric caffeine dehydrogenase was purified from Pseudomonas sp. strain CBB1. This enzyme oxidized caffeine to trimethyluric acid stoichiometrically and hydrolytically, without producing hydrogen peroxide. The enzyme was not NAD(P)+ dependent; coenzyme Q0 was the preferred electron acceptor. The enzyme was specific for caffeine and theobromine and showed no activity with xanthine.

* Corresponding author. Mailing address: Center for Biocatalysis & Bioprocessing, The University of Iowa, Oakdale Research Park, 2501 Crosspark Road, Suite C100, Coralville, IA 52241. Phone: (319) 335-4900. Fax: (315) 339-4901. E-mail: mani-subramanian{at}uiowa.edu

{triangledown} Published ahead of print on 2 November 2007.

Journal of Bacteriology, January 2008, p. 772-776, Vol. 190, No. 2
0021-9193/08/$08.00+0     doi:10.1128/JB.01390-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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