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Journal of Bacteriology, October 2008, p. 6922-6926, Vol. 190, No. 20
0021-9193/08/$08.00+0     doi:10.1128/JB.00934-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Interaction of Penicillin-Binding Protein 2 with Soluble Lytic Transglycosylase B1 in Pseudomonas aeruginosa{triangledown}

Blaine A. Legaree and Anthony J. Clarke*

Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada

Received 8 July 2008/ Accepted 31 July 2008

Soluble lytic transglycosylase B1 from Pseudomonas aeruginosa was coupled to Sepharose and used to immobilize interaction partners from membrane protein extracts. Penicillin-binding protein 2 (PBP2) was identified as a binding partner, suggesting that the two proteins function together in the biosynthesis of peptidoglycan. By use of an engineered truncated derivative, the N-terminal module of PBP2 was found to confer the binding properties.

* Corresponding author. Mailing address: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Phone: (519) 824-4120. Fax: (519) 837-1802. E-mail: aclarke{at}uoguelph.ca

{triangledown} Published ahead of print on 15 August 2008.

Journal of Bacteriology, October 2008, p. 6922-6926, Vol. 190, No. 20
0021-9193/08/$08.00+0     doi:10.1128/JB.00934-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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