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Journal of Bacteriology, November 2008, p. 7298-7301, Vol. 190, No. 21
0021-9193/08/$08.00+0     doi:10.1128/JB.00551-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Involvement of an Essential Gene, mviN, in Murein Synthesis in Escherichia coli{triangledown} ,{dagger}

Azusa Inoue,1 Yoshimitsu Murata,2 Hiroshi Takahashi,2 Naoko Tsuji,1 Shingo Fujisaki,2 and Jun-ichi Kato1*

Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan,1 Department of Biomolecular Science, Faculty of Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan2

Received 22 April 2008/ Accepted 4 August 2008

We isolated a temperature-sensitive mutant with a mutation in mviN, an essential gene in Escherichia coli. At the nonpermissive temperature, mviN mutant cells swelled and burst. An intermediate in murein synthesis, polyprenyl diphosphate-N-acetylmuramic acid-(pentapeptide)-N-acetyl-glucosamine, accumulated in mutant cells. These results indicated that MviN is involved in murein synthesis.

* Corresponding author. Mailing address: Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan. Phone: 81-42-677-2569. Fax: 81-42-677-2569. E-mail: jkato{at}tmu.ac.jp

{triangledown} Published ahead of print on 15 August 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2008, p. 7298-7301, Vol. 190, No. 21
0021-9193/08/$08.00+0     doi:10.1128/JB.00551-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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