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Journal of Bacteriology, December 2008, p. 7655-7665, Vol. 190, No. 23
0021-9193/08/$08.00+0     doi:10.1128/JB.01489-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Two Homologous Agr-Like Quorum-Sensing Systems Cooperatively Control Adherence, Cell Morphology, and Cell Viability Properties in Lactobacillus plantarum WCFS1{triangledown}

Toshio Fujii,1,2* Colin Ingham,1 Jiro Nakayama,3 Marke Beerthuyzen,4 Ryoko Kunuki,3 Douwe Molenaar,4 Mark Sturme,1 Elaine Vaughan,1,{dagger} Michiel Kleerebezem,1,4,5 and Willem de Vos1,5,{ddagger}

Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands,1 Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd., Yokohama, Japan,2 Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan,3 NIZO Food Research, Ede, The Netherlands,4 Top Institute Food and Nutrition, Wageningen, The Netherlands5

Received 14 September 2007/ Accepted 9 September 2008

A two-component regulatory system of Lactobacillus plantarum, encoded by genes designated lamK and lamR (hpk10 and rrp10), was studied. The lamK and lamR genes encode proteins which are highly homologous to the quorum-sensing histidine kinase LamC and the response regulator LamA, respectively. Transcription analysis of the lamKR operon and the lamBDCA operon and liquid chromatography-mass spectrometry analysis of production of the LamD558 autoinducing peptide were performed for {Delta}lamA, {Delta}lamR, {Delta}lamA {Delta}lamR deletion mutants and a wild-type strain. The results suggested that lamA and lamR are cooperating genes. In addition, typical phenotypes of the {Delta}lamA mutant, such as reduced adherence to glass surfaces and filamentous cell morphology, were enhanced in the {Delta}lamA {Delta}lamR mutant. Microarray analysis suggested that the same cell wall polysaccharide synthesis genes, stress response-related genes, and cell wall protein-encoding genes were affected in the {Delta}lamA and {Delta}lamA {Delta}lamR mutants. However, the regulation ratio was more significant for the {Delta}lamA {Delta}lamR mutant, indicating the cooperative effect of LamA and LamR.

* Corresponding author. Mailing address: Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd., 1-13-5, Fukuura Kanazawa-ku, Yokohama 236-0004, Japan. Phone: 81-45-330-9005. Fax: 81-45-788-4047. E-mail: tfujii{at}kirin.co.jp

{triangledown} Published ahead of print on 19 September 2008.

{dagger} Present address: Unilever Food & Health Research Institute, Unilever R&D, Olivier van Noortlaan 120, 3133 AT Vlaardingen, The Netherlands.

{ddagger} Present address: Department of Basic Veterinary Sciences, FI-00014 University of Helsinki, Helsinki, Finland.

Journal of Bacteriology, December 2008, p. 7655-7665, Vol. 190, No. 23
0021-9193/08/$08.00+0     doi:10.1128/JB.01489-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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