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Journal of Bacteriology, December 2008, p. 7762-7772, Vol. 190, No. 23
0021-9193/08/$08.00+0     doi:10.1128/JB.01032-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of a Repressor of a Truncated Denitrification Pathway in Moraxella catarrhalis{triangledown}

Wei Wang,1 Anthony R. Richardson,2 Willm Martens-Habbena,3 David A. Stahl,3 Ferric C. Fang,2 and Eric J. Hansen1*

Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390,1 Department of Laboratory Medicine,2 Department of Civil and Environmental Engineering, University of Washington, Seattle, Washington 981953

Received 25 July 2008/ Accepted 17 September 2008

Growth of Moraxella catarrhalis in a biofilm resulted in marked upregulation of two open reading frames (ORFs), aniA and norB, predicted to encode a nitrite reductase and a nitric oxide reductase, respectively (W. Wang, L. Reitzer, D. A. Rasko, M. M. Pearson, R. J. Blick, C. Laurence, and E. J. Hansen, Infect. Immun. 75:4959-4971, 2007). An ORF designated nsrR, which was located between aniA and norB, was shown to encode a predicted transcriptional regulator. Inactivation of nsrR resulted in increased expression of aniA and norB in three different M. catarrhalis strains, as measured by both DNA microarray analysis and quantitative reverse transcriptase PCR. Provision of a wild-type nsrR gene in trans in an nsrR mutant resulted in decreased expression of the AniA protein. DNA microarray analysis revealed that two other ORFs (MC ORF 683 and MC ORF 1550) were also consistently upregulated in an nsrR mutant. Consumption of both nitrite and nitric oxide occurred more rapidly with cells of an nsrR mutant than with wild-type cells. However, growth of nsrR mutants was completely inhibited by a low level of sodium nitrite. This inhibition of growth by nitrite was significantly reversed by introduction of an aniA mutation into the nsrR mutant and was completely reversed by the presence of a wild-type nsrR gene in trans. NsrR regulation of the expression of aniA was sensitive to nitrite, whereas NsrR regulation of norB was sensitive to nitric oxide.

* Corresponding author. Mailing address: Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9048. Phone: (214) 648-5974. Fax: (214) 648-5905. E-mail: eric.hansen{at}utsouthwestern.edu

{triangledown} Published ahead of print on 26 September 2008.

Journal of Bacteriology, December 2008, p. 7762-7772, Vol. 190, No. 23
0021-9193/08/$08.00+0     doi:10.1128/JB.01032-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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