This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adams, M. D.
Right arrow Articles by Gill, S. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adams, M. D.
Right arrow Articles by Gill, S. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2008, p. 8053-8064, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00834-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Comparative Genome Sequence Analysis of Multidrug-Resistant Acinetobacter baumannii{triangledown} ,{dagger}

Mark D. Adams,1* Karrie Goglin,1,{ddagger} Neil Molyneaux,1 Kristine M. Hujer,2 Heather Lavender,1 Jennifer J. Jamison,3 Ian J. MacDonald,3 Kristienna M. Martin,3 Thomas Russo,3,4,5,7 Anthony A. Campagnari,3,7 Andrea M. Hujer,2 Robert A. Bonomo,2,6 and Steven R. Gill3,8

Department of Genetics,1 Departments of Pharmacology and Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio,6 Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio,2 New York State Center of Excellence in Bioinformatics and Life Sciences,3 Veterans Administration Western New York Healthcare System,4 Department of Medicine,5 Department of Microbiology & Immunology,7 Department of Oral Biology, The State University of New York at Buffalo, Buffalo, New York8

Received 16 June 2008/ Accepted 6 October 2008

The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii has raised concern in health care settings worldwide. In order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three MDR and three drug-susceptible A. baumannii isolates. The entire MDR phenotype can be explained by the acquisition of discrete resistance determinants distributed throughout the genome. A comparison of closely related MDR and drug-susceptible isolates suggests that drug efflux may be a less significant contributor to resistance to certain classes of antibiotics than inactivation enzymes are. A resistance island with a variable composition of resistance determinants interspersed with transposons, integrons, and other mobile genetic elements is a significant but not universal contributor to the MDR phenotype. Four hundred seventy-five genes are shared among all six clinical isolates but absent from the related environmental species Acinetobacter baylyi ADP1. These genes are enriched for transcription factors and transporters and suggest physiological features of A. baumannii that are related to adaptation for growth in association with humans.

* Corresponding author. Mailing address: Department of Genetics, Case Western Reserve University School of Medicine, 10900 Euclid Ave., Cleveland, OH 44106-4955. Phone: (216) 368-2791. Fax: (216) 368-3432. E-mail: mda13{at}case.edu

{triangledown} Published ahead of print on 17 October 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Scripps Healthcare, San Diego, CA.

Journal of Bacteriology, December 2008, p. 8053-8064, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00834-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Post, V., Hall, R. M. (2009). AbaR5, a Large Multiple-Antibiotic Resistance Region Found in Acinetobacter baumannii. Antimicrob. Agents Chemother. 53: 2667-2671 [Abstract] [Full Text]  
  • Mugnier, P. D., Poirel, L., Nordmann, P. (2009). Functional Analysis of Insertion Sequence ISAba1, Responsible for Genomic Plasticity of Acinetobacter baumannii. J. Bacteriol. 191: 2414-2418 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»