This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cooke, F. J.
Right arrow Articles by Dougan, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cooke, F. J.
Right arrow Articles by Dougan, G.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2008, p. 8155-8162, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00636-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of the Genomes of a Diverse Collection of Salmonella enterica Serovar Typhimurium Definitive Phage Type 104{triangledown}

Fiona J. Cooke,1* Derek J. Brown,2 Maria Fookes,1 Derek Pickard,1 Alasdair Ivens,1 John Wain,1 Mark Roberts,3 Robert A. Kingsley,1 Nicholas R. Thomson,1 and Gordon Dougan1

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom,1 Scottish Salmonella Reference Laboratory, Stobhill Hospital, 133 Balornock Road, Glasgow G21 3UW, Scotland,2 Faculty of Veterinary Medicine, 464 Bearsden Road, Glasgow G61 1QH, Scotland3

Received 7 May 2008/ Accepted 9 September 2008

Salmonella enterica serovar Typhimurium definitive phage type 104 (DT104) has caused significant morbidity and mortality in humans and animals for almost three decades. We completed the full DNA sequence of one DT104 strain, NCTC13348, and showed that significant differences between the genome of this isolate and the genome of the previously sequenced strain Salmonella serovar Typhimurium LT2 are due to integrated prophage elements and Salmonella genomic island 1 encoding antibiotic resistance genes. Thirteen isolates of Salmonella serovar Typhimurium DT104 with different pulsed-field gel electrophoresis (PFGE) profiles were analyzed by using multilocus sequence typing (MLST), plasmid profiling, hybridization to a pan-Salmonella DNA microarray, and prophage-based multiplex PCR. All the isolates belonged to a single MLST type, sequence type ST19. Microarray data demonstrated that the gene contents of the 13 DT104 isolates were remarkably conserved. The PFGE DNA fragment size differences in these isolates could be explained to a great extent by differences in the prophage and plasmid contents. Thus, here the nature of variation in different Salmonella serovar Typhimurium DT104 isolates is further defined at the gene and whole-genome levels, illustrating how this phage type evolves over time.

* Corresponding author. Mailing address: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom. Phone: 44 (0) 1223 495398. Fax: 44 (0) 1223494919. E-mail: fiona{at}sanger.ac.uk

{triangledown} Published ahead of print on 10 October 2008.

Journal of Bacteriology, December 2008, p. 8155-8162, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00636-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Soyer, Y., Moreno Switt, A., Davis, M. A., Maurer, J., McDonough, P. L., Schoonmaker-Bopp, D. J., Dumas, N. B., Root, T., Warnick, L. D., Grohn, Y. T., Wiedmann, M. (2009). Salmonella enterica Serotype 4,5,12:i:-, an Emerging Salmonella Serotype That Represents Multiple Distinct Clones. J. Clin. Microbiol. 47: 3546-3556 [Abstract] [Full Text]  
  • Djordjevic, S. P., Cain, A. K., Evershed, N. J., Falconer, L., Levings, R. S., Lightfoot, D., Hall, R. M. (2009). Emergence and Evolution of Multiply Antibiotic-Resistant Salmonella enterica Serovar Paratyphi B D-Tartrate-Utilizing Strains Containing SGI1. Antimicrob. Agents Chemother. 53: 2319-2326 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»