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Journal of Bacteriology, December 2008, p. 8215-8219, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.01009-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of Bdellovibrio bacteriovorus HD100 Bd0714 as a Nudix dGTPase{triangledown} ,{dagger}

Susan R. Steyert,1,§ Simon A. J. Messing,2,§ L. Mario Amzel,2 Sandra B. Gabelli,2 and Silvia A. Piñeiro1*

Department of Medical and Research Technology, School of Medicine, University of Maryland, Baltimore,1 Department of Biophysics and Biophysical Chemistry, School of Medicine, Johns Hopkins University, Baltimore, Maryland2

Received 22 July 2008/ Accepted 9 October 2008

Bdellovibrio bacteriovorus bacteria are predatory organisms that attack other gram-negative bacteria. Here, we report that Bd0714 is a Nudix dGTPase from B. bacteriovorus HD100 with a substrate specificity similar to that of Escherichia coli MutT and complements an E. coli mutT-deficient strain. We observed different transcription levels of the gene throughout the predator life cycle.


* Corresponding author. Mailing address: Department of Medical and Research Technology, School of Medicine, University of Maryland, Baltimore, 100 Penn Street-AHB-Office 415A-Lab 450, Baltimore, MD 21201. Phone: (410) 706-3773. Fax: (410) 706-0073. E-mail: spineiro{at}som.umaryland.edu

{triangledown} Published ahead of print on 17 October 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

§ These authors contributed equally to this paper.


Journal of Bacteriology, December 2008, p. 8215-8219, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.01009-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.