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Journal of Bacteriology, December 2008, p. 8223-8224, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00908-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Bacterial Flagella Are Firmly Anchored{triangledown}

Nicholas C. Darnton{dagger} and Howard C. Berg*

Rowland Institute at Harvard, Cambridge, Massachusetts 02142, and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138

Received 1 July 2008/ Accepted 29 September 2008

There are mutants of Salmonella enterica (with mutations in fliF and fliL) that shed flagella when they are swimming in a viscous medium or on the surface of soft agar. Filaments with hooks and the distal rod segment FlgG are recovered. We tried to extract flagellar filaments from such cells by pulling on them with an optical trap but failed, even when we used forces large enough to straighten the filaments. Thus, flagella are firmly anchored.

* Corresponding author. Mailing address: Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138. Phone: (617) 495-0924. Fax: (617) 496-1114. E-mail: hberg{at}mcb.harvard.edu

{triangledown} Published ahead of print on 10 October 2008.

{dagger} Present address: Department of Physics, Amherst College, Amherst, MA 01002.

Journal of Bacteriology, December 2008, p. 8223-8224, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00908-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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