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Journal of Bacteriology, December 2008, p. 8225-8229, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00912-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Site-Directed Mutagenesis Reveals Putative Substrate Binding Residues in the Escherichia coli RND Efflux Pump AcrB{triangledown}

Jürgen A. Bohnert,1* Sabine Schuster,1 Markus A. Seeger,2 Eva Fähnrich,1 Klaas M. Pos,2 and Winfried V. Kern1

Center for Infectious Diseases and Travel Medicine, University Hospital, and Department of Medicine, Albert Ludwigs University, Freiburg, Germany,1 Institute of Physiology and Zürich Centre for Integrative Human Physiology, University of Zürich, Zürich, Switzerland2

Received 2 July 2008/ Accepted 1 October 2008

The Escherichia coli multidrug efflux pump protein AcrB has recently been cocrystallized with various substrates, suggesting that there is a phenylalanine-rich binding site around F178 and F615. We found that F610A was the point mutation that had the most significant impact on substrate MICs, while other targeted mutations, including conversion of phenylalanines 136, 178, 615, 617, and 628 to alanine, had smaller and more variable effects.

* Corresponding author. Mailing address: Medizinische Universitätsklinik, Hugstetter Strasse 55, D-79106 Freiburg, Germany. Phone: 49-761-270 1819. Fax: 49-761-270 1820. E-mail: juergen{at}bohnert.name

{triangledown} Published ahead of print on 10 October 2008.

Journal of Bacteriology, December 2008, p. 8225-8229, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00912-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Symmons, M. F., Bokma, E., Koronakis, E., Hughes, C., Koronakis, V. (2009). From the Cover: The assembled structure of a complete tripartite bacterial multidrug efflux pump. Proc. Natl. Acad. Sci. USA 106: 7173-7178 [Abstract] [Full Text]  
  • Wehmeier, C., Schuster, S., Fahnrich, E., Kern, W. V., Bohnert, J. A. (2009). Site-Directed Mutagenesis Reveals Amino Acid Residues in the Escherichia coli RND Efflux Pump AcrB That Confer Macrolide Resistance. Antimicrob. Agents Chemother. 53: 329-330 [Full Text]  


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