JB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JB.01488-07v1
190/4/1484    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gradman, R. J.
Right arrow Articles by Reznikoff, W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gradman, R. J.
Right arrow Articles by Reznikoff, W. S.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2008, p. 1484-1487, Vol. 190, No. 4
0021-9193/08/$08.00+0     doi:10.1128/JB.01488-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Tn5 Synaptic Complex Formation: Role of Transposase Residue W450{triangledown}

Richard J. Gradman1 and William S. Reznikoff1,2*

Department of Biochemistry, University of Wisconsin—Madison, Madison, Wisconsin 53706,1 The Josephine Bay Paul Center, The Marine Biology Laboratory, Woods Hole, Massachusetts 025432

Received 14 September 2007/ Accepted 4 December 2007

A series of Tn5 transposases (Tnp's) with mutations at the conserved amino acid position W450, which was structurally predicted to be important for synapsis, have been generated and characterized. This study demonstrates that W450 is involved in hydrophobic (and possibly aromatic) contacts within the Tnp monomer that negatively regulate synaptic complex formation.

* Corresponding author. Mailing address: The Josephine Bay Paul Center, Marine Biological Laboratory, 7 MBL St., Woods Hole, MA 02543. Phone: (508) 289-7253. Fax: (508) 457-4727. E-mail: wsreznik{at}wisc.edu

{triangledown} Published ahead of print on 14 December 2007.

Journal of Bacteriology, February 2008, p. 1484-1487, Vol. 190, No. 4
0021-9193/08/$08.00+0     doi:10.1128/JB.01488-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 2008 by the American Society for Microbiology. All rights reserved.