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DNA Microarray Analysis of Central Carbohydrate Metabolism: Glycolytic/Gluconeogenic Carbon Switch in the Hyperthermophilic Crenarchaeum Thermoproteus tenax ^,

Melanie Zaparty,^1* Alexander Zaigler,^2, Claudia Stamme,² Jörg Soppa,² Reinhard Hensel,¹ and Bettina Siebers¹

Department of Biology and Geography, Institute of Biology, Microbiology I, University of Duisburg-Essen, Universitätsstr. 5, 45117 Essen, Germany,¹ Biocenter Niederursel, Institute of Microbiology, Goethe-University, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany²

Received 21 September 2007/ Accepted 23 December 2007

In order to unravel the role of regulation on transcript level in central carbohydrate metabolism (CCM) of Thermoproteus tenax, a focused DNA microarray was constructed by using 85 open reading frames involved in CCM. A transcriptional analysis comparing heterotrophic growth on glucose versus autotrophic growth on CO₂-H₂ was performed.

Published ahead of print on 4 January 2008.

Supplemental material for this article may be found at http://jb.asm.org/.

Present address: GANYMED Pharmaceuticals, Freiligrathstr. 12, 55131 Mainz, Germany.