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Polarity in Archaeal Operon Transcription in Thermococcus kodakaraensis

Thomas J. Santangelo,¹ L'ubomíra uboová,¹ Rie Matsumi,² Haruyuki Atomi,² Tadayuki Imanaka,² and John N. Reeve^1*

Department of Microbiology, Ohio State University, Columbus, Ohio 43210,¹ Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan²

Received 15 November 2007/ Accepted 2 January 2008

An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.

Published ahead of print on 11 January 2008.

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