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Journal of Bacteriology, March 2008, p. 2244-2248, Vol. 190, No. 6
0021-9193/08/$08.00+0 doi:10.1128/JB.01811-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

ubo
ová,1
Rie Matsumi,2
Haruyuki Atomi,2
Tadayuki Imanaka,2 and
John N. Reeve1*
Department of Microbiology, Ohio State University, Columbus, Ohio 43210,1 Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan2
Received 15 November 2007/ Accepted 2 January 2008
An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.
Published ahead of print on 11 January 2008.
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