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Journal of Bacteriology, March 2008, p. 2244-2248, Vol. 190, No. 6
0021-9193/08/$08.00+0     doi:10.1128/JB.01811-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Polarity in Archaeal Operon Transcription in Thermococcus kodakaraensis{triangledown}

Thomas J. Santangelo,1 L'ubomíra Cubonová,1 Rie Matsumi,2 Haruyuki Atomi,2 Tadayuki Imanaka,2 and John N. Reeve1*

Department of Microbiology, Ohio State University, Columbus, Ohio 43210,1 Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto 615-8510, Japan2

Received 15 November 2007/ Accepted 2 January 2008

An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential β-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.

* Corresponding author. Mailing address: Department of Microbiology, Ohio State University, Columbus, OH 43210-1292. Phone: (614) 292-2301. Fax: (614) 292-8120. E-mail: reeve.2{at}osu.edu

{triangledown} Published ahead of print on 11 January 2008.

Journal of Bacteriology, March 2008, p. 2244-2248, Vol. 190, No. 6
0021-9193/08/$08.00+0     doi:10.1128/JB.01811-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Santangelo, T. J., Cubonova, L., Skinner, K. M., Reeve, J. N. (2009). Archaeal Intrinsic Transcription Termination In Vivo. J. Bacteriol. 191: 7102-7108 [Abstract] [Full Text]  
  • Santangelo, T. J., Cubonova, L., Reeve, J. N. (2008). Shuttle Vector Expression in Thermococcus kodakaraensis: Contributions of cis Elements to Protein Synthesis in a Hyperthermophilic Archaeon. Appl. Environ. Microbiol. 74: 3099-3104 [Abstract] [Full Text]  


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