This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pickard, D.
Right arrow Articles by Dougan, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pickard, D.
Right arrow Articles by Dougan, G.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2008, p. 2580-2587, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01654-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of the Salmonella enterica Serovar Typhi Vi-Typing Bacteriophage E1{triangledown}

Derek Pickard,1* Nicholas R. Thomson,1 Stephen Baker,2 John Wain,1 Mercedes Pardo,1 David Goulding,1 Nancy Hamlin,1 Jyoti Choudhary,1 John Threfall,3 and Gordon Dougan1

The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom,1 Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, Quan 5, Ho Chi Minh City, Vietnam,2 Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, United Kingdom3

Received 12 October 2007/ Accepted 28 December 2007

Some bacteriophages target potentially pathogenic bacteria by exploiting surface-associated virulence factors as receptors. For example, phage have been identified that exhibit specificity for Vi capsule producing Salmonella enterica serovar Typhi. Here we have characterized the Vi-associated E1-typing bacteriophage using a number of molecular approaches. The absolute requirement for Vi capsule expression for infectivity was demonstrated using different Vi-negative S. enterica derivatives. The phage particles were shown to have an icosahedral head and a long noncontractile tail structure. The genome is 45,362 bp in length with defined capsid and tail regions that exhibit significant homology to the S. enterica transducing phage ES18. Mass spectrometry was used to confirm the presence of a number of hypothetical proteins in the Vi phage E1 particle and demonstrate that a number of phage proteins are modified posttranslationally. The genome of the Vi phage E1 is significantly related to other bacteriophages belonging to the same serovar Typhi phage-typing set, and we demonstrate a role for phage DNA modification in determining host specificity.

* Corresponding author. Mailing address: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom. Phone: 441223495391. Fax: 441223494919. E-mail: djp{at}sanger.ac.uk

{triangledown} Published ahead of print on 11 January 2008.

Journal of Bacteriology, April 2008, p. 2580-2587, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01654-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»