This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walter, B.
Right arrow Articles by Burkovski, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walter, B.
Right arrow Articles by Burkovski, A.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2008, p. 2611-2614, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01896-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Dissection of Ammonium Uptake Systems in Corynebacterium glutamicum: Mechanism of Action and Energetics of AmtA and AmtB{triangledown}

Britta Walter,1 Melanie Küspert,1 Daniel Ansorge,2 Reinhard Krämer,2* and Andreas Burkovski1*

Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany,1 Institut für Biochemie der Universität zu Köln, Zülpicher-Str. 47, 50674 Köln, Germany2

Received 4 December 2007/ Accepted 18 January 2008

Corynebacterium glutamicum has two different Amt-type proteins. While AmtB has a low substrate affinity and is not saturable up to 3 mM methylammonium, AmtA has a high substrate affinity and mediates saturable, membrane potential-dependent transport, resulting in a high steady-state accumulation of methylammonium, even in the absence of metabolic trapping.

* Corresponding author. Mailing address for Reinhard Krämer: Institut für Biochemie, Universität zu Köln, Zülpicher-Str. 47, 50674 Köln, Germany. Phone: 49 221 470 6461. Fax: 49 221 470 5091. E-mail: r.kraemer{at}uni-koeln.de. Mailing address for Andreas Burkovski: Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. Phone: 49 9131 85 28086. Fax: 49 9131 85 28082. E-mail: aburkov{at}biologie.uni-erlangen.de

{triangledown} Published ahead of print on 1 February 2008.

Journal of Bacteriology, April 2008, p. 2611-2614, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01896-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»