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RecO Is Essential for DNA Damage Repair in Deinococcus radiodurans

Guangzhi Xu,^1, Liangyan Wang,^1,2, Huan Chen,^1,2,3 Huiming Lu,¹ Nanjiao Ying,¹ Bing Tian,¹ and Yuejin Hua^1*

Key Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear-Agricultural Sciences, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China,¹ College of Life Sciences, Zhejiang University, Hangzhou 310029, China,² James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310008, China³

Received 25 November 2007/ Accepted 13 January 2008

Here we present direct evidence for the vital role of RecO in Deinococcus radiodurans's radioresistance. A recO null mutant was constructed using a deletion replacement method. The mutant exhibited a growth defect and extreme sensitivity to irradiation with gamma rays and UV light. These results suggest that DNA repair in this organism occurs mainly via the RecF pathway.

Published ahead of print on 25 January 2008.

Guangzhi Xu and Liangyan Wang contributed equally to this work.