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TrpY Regulation of trpB2 Transcription in Methanothermobacter thermautotrophicus

Elizabeth A. Karr,^1, Kathleen Sandman,¹ Rudi Lurz,² and John N. Reeve^1*

Department of Microbiology, Ohio State University, Columbus, Ohio 43210,¹ Max-Planck-Institut für Molekulare Genetik, D-14195 Berlin, Germany²

Received 11 December 2007/ Accepted 25 January 2008

TrpY binds specifically to TRP box sequences upstream of trpB2, but the repression of trpB2 transcription requires additional TrpY assembly that is stimulated by but not dependent on the presence of tryptophan. Inhibitory complex formation is prevented by insertions within the regulatory region and by a G149R substitution in TrpY, even though TrpY(G149R) retains both TRP box DNA- and tryptophan-binding abilities.

Published ahead of print on 8 February 2008.

Present address: Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019.