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Journal of Bacteriology, April 2008, p. 2804-2813, Vol. 190, No. 8
0021-9193/08/$08.00+0     doi:10.1128/JB.01572-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

In Vivo Growth of Pseudomonas aeruginosa Strains PAO1 and PA14 and the Hypervirulent Strain LESB58 in a Rat Model of Chronic Lung Infection{triangledown}

Irena Kukavica-Ibrulj,1 Alessandra Bragonzi,2 Moira Paroni,2 Craig Winstanley,4 François Sanschagrin,1 George A. O'Toole,3 and Roger C. Levesque1*

Centre de Recherche sur la Fonction, Structure et Ingénierie des Protéines, Pavillon Charles-Eugène Marchand, Faculté de Médecine, Université Laval, Laval, Québec G1K 7P4, Canada,1 Institute for Experimental Treatment of Cystic Fibrosis, HS Raffaele Scientific Institute, Milan, Italy,2 Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire 03755,3 Division of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom4

Received 28 September 2007/ Accepted 23 November 2007

Pseudomonas aeruginosa chronic lung infections are the major cause of morbidity and mortality in cystic fibrosis (CF) patients. The P. aeruginosa strains PAO1 and PA14 were compared with the Liverpool epidemic strain LESB58 to assess in vivo growth, infection kinetics, and bacterial persistence and localization within tissues in a rat model of chronic lung infection. The three P. aeruginosa strains demonstrated similar growth curves in vivo but differences in tissue distribution. The LESB58 strain persisted in the bronchial lumen, while the PAO1 and PA14 strains were found localized in the alveolar regions and grew as macrocolonies after day 7 postinfection. Bacterial strains were compared for swimming and twitching motility and for the production of biofilm. The P. aeruginosa LESB58 strain produced more biofilm than PAO1 and PA14. Competitive index (CI) analysis of PAO1, PA14, and LESB58 in vivo indicated CI values of 0.002, 0.0002, and 0.14 between PAO1-PA14, PAO1-LESB58, and LESB58-PA14, respectively. CI analysis comparing the in vivo growth of the PAO1 {Delta}PA5441 mutant and four PA14 surface attachment-defective (sad) mutants gave CI values 10 to 1,000 times lower in competitions with their respective wild-type strains PAO1 and PA14. P. aeruginosa strains studied in the rat model of chronic lung infection demonstrated similar in vivo growth but differences in virulence as shown with a competitive in vivo assay. These differences were further confirmed with biofilm and motility in vitro assays, where strain LESB58 produced more biofilm but had less capacity for motility than PAO1 and PA14.

* Corresponding author. Mailing address: Centre de Recherche sur la Fonction, Structure et Ingénierie des Protéines, Pavillon Charles-Eugène Marchand, Biologie Médicale, Faculté de Médecine, Université Laval, Laval, Québec G1K 7P4, Canada. Phone: (418) 656-2131, ext. 4471. Fax: (418) 656-7176. E-mail: rclevesq{at}rsvs.ulaval.ca

{triangledown} Published ahead of print on 14 December 2007.

Journal of Bacteriology, April 2008, p. 2804-2813, Vol. 190, No. 8
0021-9193/08/$08.00+0     doi:10.1128/JB.01572-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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