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Journal of Bacteriology, April 2008, p. 3057-3062, Vol. 190, No. 8
0021-9193/08/$08.00+0     doi:10.1128/JB.01700-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

YjgF Is Required for Isoleucine Biosynthesis when Salmonella enterica Is Grown on Pyruvate Medium{triangledown}

Melissa R. Christopherson, G. E. Schmitz, and Diana M. Downs*

Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 23 October 2007/ Accepted 11 February 2008

The YjgF/YER057c/UK114 family of proteins is conserved across the three domains of life, yet no biochemical function has been clearly defined for any member of this family. In Salmonella enterica, a deletion of yjgF results in a requirement for isoleucine when the mutant strain is grown in glucose-serine or pyruvate medium. Feedback inhibition of IlvA is required for the curative effect of isoleucine on glucose-serine medium. On pyruvate medium, yjgF mutants are unable to synthesize enough isoleucine for growth. From this study, we conclude that the isoleucine requirement of a yjgF mutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity that has previously been characterized in these mutants.

* Corresponding author. Mailing address: Department of Bacteriology, University of Wisconsin—Madison, 1550 Linden Dr., Madison, WI 53706. Phone: (608) 238-0383. Fax: (608) 262-9865. E-mail: downs{at}bact.wisc.edu

{triangledown} Published ahead of print on 22 February 2008.

Journal of Bacteriology, April 2008, p. 3057-3062, Vol. 190, No. 8
0021-9193/08/$08.00+0     doi:10.1128/JB.01700-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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