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Journal of Bacteriology, April 2008, p. 3057-3062, Vol. 190, No. 8
0021-9193/08/$08.00+0     doi:10.1128/JB.01700-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

YjgF Is Required for Isoleucine Biosynthesis when Salmonella enterica Is Grown on Pyruvate Medium

Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 23 October 2007/ Accepted 11 February 2008

The YjgF/YER057c/UK114 family of proteins is conserved across the three domains of life, yet no biochemical function has been clearly defined for any member of this family. In Salmonella enterica, a deletion of yjgF results in a requirement for isoleucine when the mutant strain is grown in glucose-serine or pyruvate medium. Feedback inhibition of IlvA is required for the curative effect of isoleucine on glucose-serine medium. On pyruvate medium, yjgF mutants are unable to synthesize enough isoleucine for growth. From this study, we conclude that the isoleucine requirement of a yjgF mutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity that has previously been characterized in these mutants.

Published ahead of print on 22 February 2008.